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🧪 Lactic acid

Nutrient ✔ Checked: 11.2026 · sources: 4 Updated: — Text expanded, FAQ and sources added

Also known as: lactic acid, E270, milk acid, 2-hydroxypropanoic acid

An organic acid that lactic acid bacteria make from lactose; it sours fermented milk and appears on labels as E270.

Lactic acid (E270, 2-hydroxypropanoic acid) is the organic acid that makes fermented milk sour. Lactic acid bacteria produce it when they ferment lactose, the milk sugar. On food labels it carries the code E270 and counts as an acidity regulator.

Here is what happens in milk. Bacteria feed on lactose and give off acid, and the pH falls. The protein casein, which is 75–80% of milk protein, stays dispersed because its particles carry a charge and push each other away. Acid cancels that charge, and near pH 4.2 the particles stick together. That is how milk thickens. A curd made by bacteria is soft and breaks up easily. The acid from a starter culture also helps expel whey and holds back harmful bacteria.

There is not much of it in the finished food. FAO gives the acidity of a finished yoghurt as usually 0.8–0.9% lactic acid. The Indian dahi should reach 0.9–1.0%. Dairy people measure acidity just this way: they titrate milk with alkali and write the result as percent lactic acid. See acidity for more on that number.

Lactose and lactic acid are easy to mix up. Lactose is already in fresh milk; the acid appears only when bacteria work on that sugar. Kefir, as FAO describes it, is made by lactic fermentation, and yoghurt by fermenting lactose with a pure culture of lactic acid bacteria. To taste the result, try kefir, yoghurt or labneh.

On a label, E270 sits among the acidity regulators. The JECFA committee of FAO and WHO evaluated it in 2001: the acceptable daily intake is not limited, and there is no safety concern at current intakes when used as a flavouring. In FAO's additive rules most food groups allow it at the level of good manufacturing practice, with numeric limits for some groups, such as 6000 mg/kg in fresh minced meat.

For acids in wine, see malolactic fermentation and tartaric acid. For souring milk at home, see acidification. The composition of milk as a whole is in the section on milk composition, health and substitutes.

Facts

Document identifierE270 (INS 270) 1
Main useAcidity regulator and flavouring agent 1
AcidityFinished yoghurt: usually 0.8–0.9% lactic acid 4
StatusAcceptable daily intake not limited (JECFA, evaluated 2001) 1

Wine

Lactic acid in wine is a soft acid that appears after malolactic fermentation. Bacteria turn sharp malic acid into it.

Grapes contain a lot of malic acid. It gives a sharpness like green apples. During malolactic fermentation, bacteria, most often Oenococcus oeni, turn it into lactic acid. This acid is milder, and the wine's overall acidity falls a little. The wine becomes rounder and smoother. Malolactic fermentation is carried out in almost all red wines and some whites, for example Chardonnay.

The same lactic acid is found in soured milk, kefir and other fermented dairy products. In wine it arises differently, but the substance is the same. Lactic acid belongs to a wine's acidity along with tartaric and malic acid. Its amount depends on whether malolactic fermentation took place. If it is not carried out, more malic acid remains, and the taste is fresher and sharper. It is one of the ways a winemaker sets the style of a wine. Lactic acid is safe and occurs in ordinary foods.

works with🍷Malolactic fermen…produce🧫lactic acid bacte…gives🧪lactosefound in🥛kefirfound in🥣yogurtcontains🫙labnehkind of🍷Aciditykind of🧪Milk composition,…linked🍷Tartaric acidrelated🍷Acidification🧪towardia.com
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How it connects to milk

🧪 Lactic acid → found in → 🥛 Kefir → made from → 🥛 Milk

How it connects to Wine

🧪 Lactic acid → kind of → 🍷 Acidity ← contains ← 🍷 Wine

Frequently asked questions

How is acidity in milk measured?

Milk is titrated with 0.1 N sodium hydroxide up to about pH 8.3, and the result is given as percent lactic acid.

How much lactic acid is in yoghurt?

FAO gives the acidity of a finished yoghurt as usually 0.8–0.9% lactic acid, and for dahi a titratable acidity of 0.9–1.0%.

Is E270 safe as an additive?

JECFA, the FAO and WHO committee, listed the acceptable daily intake as not limited in 1973, and its 2001 evaluation saw no safety concern at current intake as a flavouring.

What does a starter culture's acid do?

FAO says it helps expel whey, speeds coagulation, shapes texture and flavour, and inhibits harmful bacteria.

Why does milk thicken when it sours?

Acid removes the charge on casein particles, so they stop repelling each other and clump. A curd made by bacteria is soft and breaks up easily.

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